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The samples were then washed 5 times for 5 min each with PBST and incubated with Hoechst 33258 at a concentration of 5 g/mL in DPBS +/+ for 15 min at RT

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[DOI] [PMC free article] [PubMed] [Google Scholar] 88.Falleti MG, Maruff P, Burman P, and Harris A